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QHow many sequencing platforms does Salus offer?

A

We offer three platforms that cover low-, medium-, and high-throughput needs: Saluseq Nimbo, Salus Pro, and Salus Evo.


QHow many sequencing units does the Salus sequenceing platforms have?

A

The Salus Pro and Salus EVO sequencer hav two units, supporting both single and dual flow cell sequencing, either simultaneously or in rolling cycles. Saluseq Nimbo supports one sequencing unit.

QWhat do the colors of the Salus sequencer status bar represent?

A

There are four colors: green, blue, yellow, and red.

Green: Standby mode;

Blue: Running normally (sequencing and cleaning stages);

Yellow: Anomalies detected—needs attention and action;

Red: Malfunction detected—requires immediate attention or contacting our engineer.

QAfter how many cycles does the Salus Pro display Q30 and cluster density?

A

After completing the 10th sequencing cycle.

QHow to clean the Salus Pro sequencer, and how long does it take?

A

The Salus Pro sequencer supportstwo washes: Fast Wash and Maintenance Wash. Fast Wash requiresthree reagents: 0.05% Tween-20, 0.1N sodium hydroxide, and lab-grade purifiedwater. Simply add all three reagents to the cleaning cartridge to start thecleaning process, which takes about 70 minutes. Maintenance Wash only useslab-grade purified water, and the cleaning process takes approximately 20minutes.

QCan the permease be mixed by shaking and brief centrifugation?

A

No, it should be mixed by pipetting or brief centrifugation.

QWhat are the technical principles behind mRNA capture with the SalusST Chip?

A

Eukaryotic mRNA is captured on the chip by oligo dT probes through binding to the poly A tail of the mRNA

QWhat are the technical principles and the roles of tissue permeabilization?

A

Permeabilization alters cellmembrane permeability, allowing mRNA to be released from the cells. Thepermeabilization test helps to determine the optimal permeabilization time foraccurate transcriptomic analysis.

QCan CY3 be used if the TRITC channel is unavailable?

A

Yes, CY3 can be used as a substitute for TRITC due to their similar excitation and emission wavelengths.

QWhat is the difference between optimization and expression chips?

A

The main difference lies in the inclusion of spatial location information. The two types of chips have different preparation processes and serve distinct purposes. Optimization chips do not contain spatial coordinate information and are only used in the early stages for determining the optimal permeabilization time. Expression chips, on the other hand, contain spatial coordinate information and can be used later for mRNA spatial localization studies.

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